AFTSpin Bacterial Fast RNA Extraction Kit (RK30123)
This kit can be used for rapid extraction of RNA from various bacterial samples, and does not rely on toxic reagents such as phenol and chloroform. The unique lysis buffer rapidly lyses cells from trace samples and inactivates cell RNA enzymes. The lysate mixture passes through a gDNA Remove Column, where gDNA is removed and RNA is filtered through. After adjusting the binding conditions by ethanol, RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt. In a series of rapid washing-centrifugation steps, the purified RNA is eluted from the silicon membrane with low salt RNase-free H2O. The extracted RNA can be directly used in RT-PCR, qPCR and RNA library constructing experiments.
AFTSpin Blood Fast RNA Extraction Kit (RK30125)
Red blood cell lysis buffer selectively lyses red blood cells, and then the unique lysis buffer / β-mercaptoethanol rapidly lyses white blood cells and inactivates cell RNA enzymes. After adjusting the binding conditions with ethanol, the RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt, and then the cell metabolites, proteins and other impurities are removed by a series of rapid washing-centrifugation steps. Finally the low-salt RNase-free H2O elutes the pure RNA from the silicon membrane.
AFTSpin Blood/Tissue/Cell Fast DNA Extraction Kit (RK30110)
This kit is suitable for quick and simple extraction of genomic DNA from various samples inclouding blood, cells and tissue. The extracted genomic DNA fragment are large, high purity and good stability, and can be directly used in PCR, enzyme digestion and hybridization experiments. The extraction process does not require phenol-chloroform extraction. Blood, cells or tissues are lysed by lysate and digested by Proteinase K, and then can be combined with Spin Column 4 under the regulation of anhydrous ethanol. After quickly and sufficiently washing, residual impurities such as protein and salt are removed, and finally DNA is dissolved in Buffer EB2.
AFTSpin Complex Plant Fast RNA Extraction Kit (RK30122)
This kit is used for RNA extraction from complex plants (polysaccharide＆polyphenol-rich plants). The kit does not rely on toxic reagents such as phenol and chloroform, and can remove residual gDNA by silica gel column purification technology and quickly extract total plant RNA. Extracted RNA can be directly used in RT-PCR, qPCR and RNA library construction experiments.
AFTSpin EndoFree Plasmid Maxi Kit (RK30103)
This kit is capable of lysing cells using a modified SDS-alkaline lysis method. The crude extract is selectively bound with a unique endotoxin scavenger and centrifuged to remove the endotoxin. The silica-based membrane in the centrifugal adsorption column can bind the plasmid DNA in the solution selectively under the condition of high salt and low pH value, and then impurities and other bacterial components can be removed by the deproteinizing solution PR and washing solution WB. Finally the low-salt, high-pH eluent EB elutes pure plasmid DNA from the silica-based membrane.
AFTSpin EndoFree Plasmid Midi Kit (RK30105)
This kit uses a modified SDS-alkaline lysis method to lyse cells. The special Buffer P4 and the Filtration Columns 1 can effectively remove impurities such as endotoxin, protein and other impurities.The kit is suitable for extracting plasmid DNA from 5-15 mL bacterial cultures, and the whole experimental procedure of plasmid DNA extraction could be finished within 1 h. Plasmid DNA prepared is suitable for a variety of downstream applications including restriction enzyme digestion, PCR, sequencing, ligation, transformation and cell transfection.
AFTSpin EndoFree Plasmid Mini Kit (RK30102)
This kit uses a modified SDS-alkaline lysis method to lyse cells. Crude extract is selectively bound to the spin column by a unique endotoxin scavenger and centrifuged to remove endotoxin. The silicone matrix membrane in the spin column selectively binds plasmid DNA at high salt and low pH conditions, allowing for impurities and other bacterial components to be removed by Buffer PR and Buffer WB. The purified plasmid DNA is eluted from the silicone matrix membrane using low-salt and high-pH Buffer EB.
AFTSpin MicroElute Fast RNA Extraction Kit (RK30124)
This kit can be used for rapid extraction of RNA from various trace samples, and does not rely on toxic reagents such as phenol and chloroform. The unique lysis buffer rapidly lyses cells from trace samples and inactivates cell RNA enzymes. The lysate mixture passes through a gDNA Remove Column, where gDNA is removed and RNA is filtered through. After adjusting the binding conditions by ethanol, RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt. In a series of rapid washing-centrifugation steps, the purified RNA is eluted from the silicon membrane with low salt RNase-free H2O. The extracted RNA can be directly used in RT-PCR, qPCR and RNA library constructing experiments.
AFTSpin Multifunction DNA Purification Kit (RK30100)
AFTSpin Multifunction DNA Purification Kit is capable of providing a simple and fast solution for gel recovery of DNA fragments and PCR product recovery, based on silica gel column purification technology. In the presence of high-disordering salts, the DNA fragments can be selectively adsorbed on the silica-based membrane in the spin column, and then the washing solution removes primers, nucleotides, proteins, enzymes, and other impurities through a series of rapid washing-centrifugation steps; the pure DNA is eluted from the silica-based membrane with a low-salt, high-pH elution buffer solution. Employing a unique gel lysis/binding solution formula, this kit integrates the two functions of gel lysis and binding as a whole, therefore one kit can be used in agarose DNA recovery, PCR product cleaning and purification, and enzyme digestion product purification and recovery. The gel lysis solution/binding solution is prepared as a yellow solution, which is convenient to observe the gel lysis effect and monitor the pH value change, thereby achieving the best binding effect and improving the recovery efficiency greatly.
AFTSpin Plasmid Mini Kit (RK30101)
The kit is capable of lysing cells using a modified SDS-alkaline lysis method. The optimized lysate allows the silica-based membrane in the centrifugal adsorption column to selectively bind the plasmid DNA in the solution under the condition of high salt and low pH value and then removes impurities and other bacterial components by deproteinizing solution PR and washing solution WB. Finally the low-salt, high-pH eluent EB elutes the pure plasmid DNA from the silica-based membrane.
In general, a high-copy plasmid is inoculated from a single colony in 1.5-4.5 mL of LB medium supplemented with appropriate antibiotics. After overnight culture for 12-16 hours, up to 20 µg of pure plasmid can be extracted.
AFTSpin Tissue/Cell Fast RNA Extraction Kit for Animal (RK30120)
This kit does not rely on toxic reagents such as phenol and chloroform, and can be used for rapid RNA extraction from common animal tissue and cell samples. The excellent silica gel column purification technology can effectively remove residual gDNA and quickly extract animal total RNA. Extracted RNA can be directly used in reverse transcription PCR, qPCR and RNA library construction experiments.