This kit is capable of lysing cells using a modified SDS-alkaline lysis method. The crude extract is selectively bound with a unique endotoxin scavenger and centrifuged to remove the endotoxin. The silica-based membrane in the centrifugal adsorption column can bind the plasmid DNA in the solution selectively under the condition of high salt and low pH value, and then impurities and other bacterial components can be removed by the deproteinizing solution PR and washing solution WB. Finally the low-salt, high-pH eluent EB elutes pure plasmid DNA from the silica-based membrane.

This kit uses a modified SDS-alkaline lysis method to lyse cells. The special Buffer P4 and the Filtration Columns 1 can effectively remove impurities such as endotoxin, protein and other impurities.The kit is suitable for extracting plasmid DNA from 5-15 mL bacterial cultures, and the whole experimental procedure of plasmid DNA extraction could be finished within 1 h. Plasmid DNA prepared is suitable for a variety of downstream applications including restriction enzyme digestion, PCR, sequencing, ligation, transformation and cell transfection.