This kit can be used for rapid extraction of RNA from various bacterial samples, and does not rely on toxic reagents such as phenol and chloroform. The unique lysis buffer rapidly lyses cells from trace samples and inactivates cell RNA enzymes. The lysate mixture passes through a gDNA Remove Column, where gDNA is removed and RNA is filtered through. After adjusting the binding conditions by ethanol, RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt. In a series of rapid washing-centrifugation steps, the purified RNA is eluted from the silicon membrane with low salt RNase-free H2O. The extracted RNA can be directly used in RT-PCR, qPCR and RNA library constructing experiments.

Red blood cell lysis buffer selectively lyses red blood cells, and then the unique lysis buffer / β-mercaptoethanol rapidly lyses white blood cells and inactivates cell RNA enzymes. After adjusting the binding conditions with ethanol, the RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt, and then the cell metabolites, proteins and other impurities are removed by a series of rapid washing-centrifugation steps. Finally the low-salt RNase-free H2O elutes the pure RNA from the silicon membrane.

This kit does not rely on toxic reagents such as phenol and chloroform, and can be used for rapid RNA extraction from common animal tissue and cell samples. The excellent silica gel column purification technology can effectively remove residual gDNA and quickly extract animal total RNA. Extracted RNA can be directly used in reverse transcription PCR, qPCR and RNA library construction experiments.