5’App-T4 DNA Ligase is a mutant of T4 DNA Ligase without adenylation function, which can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of DNA. This enzyme does not require ATP as a cofactor for ligation, but requires a pre-adenylated substrate. This enzyme reduces background ligation (chimera formation) during NGS library construction, because it can only use 5 'preadenylation adapter to the DNA fragments.

E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5′-phosphate and the 3′-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated. E. coli DNA Ligase is active at a range of temperatures (4°C – 37°C).

PBCV-1 DNA Ligase, also known as PBCV DNA Ligase, SplintR™ Ligase, or Chorella virus DNA Ligase, is an ATP-dependent DNA ligase that efficiently catalyzes the joining of two adjacent DNA single strands using a complementary RNA strand as a splint. It remains active between 16-37°C. Common applications include the detection of small RNAs such as microRNAs using probe-based methods, utilizing DNA probes to connect and detect specific RNAs, SNP or alternative splicing detection, and RASL-seq analysis.

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold).
As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.

T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It can catalyze the formation of a phosphodiester bond between adjacent 5′ phosphate and 3′ hydroxyl termini in double-stranded DNA. T7 DNA ligase can catalyze ligation of cohesive ends and repair nicks, but not blunt end ligation. This makes a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined .

Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The target strands need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37°C–75°C).
It is applicable to Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction, as well as Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification.