Mth RNA Ligase (RK20515)

Mth RNA Ligase is an ATP-dependent ligase that can efficiently convert 5′-phosphorylated single-stranded DNA into 5′-adenylated DNA. The kit has been optimized to yield high quantities of adenylylated DNA regardless of whether the 3′ end of the single-stranded DNA has been aminated or otherwise modified. Typically, the modification efficiency can reach over 95%.

T4 RNA Ligase 1 (RK20503)

T4 RNA ligase 1 catalyzes the formation of a 3´ → 5´ phosphodiester bond by joining the 5´ phosphate end of a nucleotide to the 3´ hydroxyl end, accompanied by the hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA, DNA, and dinucleoside pyrophosphates.

T4 RNA Ligase 2, truncated (RK20505)

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2 truncated is expressed from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2. Unlike the full length ligase, T4 Rnl2 truncated is unable to adenylate the 5´ end of the substrate, and as a result it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA. This enzyme, also known as Rnl2 (1-249) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers.

T4 RNA Ligase 2, truncated KQ (RK20504)

T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q), specifically catalyzes the ligation of a 5´-adenylated DNA or RNA to the 3´ OH end of an RNA molecule. This enzyme-mediated ligation does not require ATP but necessitates a pre-adenylated adapter. T4 RNA Ligase 2, truncated KQ, a double-point mutant of T4 RNA Ligase 2 with R55K and K227Q substitutions, significantly reduces the non-specific ligation of RNA (such as concatenation or self-circularization) compared to T4 RNA Ligase 2, truncated, while maintaining enzyme activity similar to that of T4 RNA Ligase 2, truncated.