DNase I , RNase-free (5,000 U/mL) (RK20549)

DNase I (deoxyribonuclease, RNase-free ) is an endonuclease that nonspecifically cleaves DNA to release di-, tri-, and oligonucleotide products with 5’phosphorylated and 3´-hydroxylated ends. The activity of DNase I depends on Ca2+ and also be activated bydivalent metal ions Mg2+, Mn2+, etc.DNase I act on various DNAs such as single and double-stranded DNA, RNA:DNA hybrids.

DNase I (Powder) (RM29865)

Deoxyribonuclease I (DNase I) is an endonuclease that nonspecifically cleaves DNA to release di-, tri-, and oligonucleotide products with 5’phosphorylated and 3´-hydroxylated ends. This product is sourced from bovine pancreas and is supplied in powder form with enzyme activity ≥ 500 Kunitz units/mg protein.

LbCas12a Nuclease (RK20570)

Lb Cas12a Nuclease from Lachnospiraceae bacterium is a site-specific DNA endonuclease guided by a single 40-44 nucleotide guide RNA (crRNA). Targeting requires a crRNA complementary to the target site as well as a 5’ TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by LbCas12a Nuclease occurs ~17 bases 3′ of the PAM and leaves overhanging ends.This product also has a trans-cleavage activity, which produces high non-specific ssDNA cleavage activity when the target dsDNA, crRNA, and Cas12a protein form a ternary complex.