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5′ Deadenylase (RK20580)

The 5′ Deadenylase derived from yeast can remove adenosine residues from the 5′-end of DNA and RNA, retaining the 5′-end phosphate group. It can cleave AppppA to produce ATP and AMP.

Formamidopyrimidine DNA Glycosylase (Fpg) (RK20520)

Formamidopyrimidine-DNA glycosylase (Fpg), also known as 8-oxoguanine DNA glycosylase, possesses two activities: N-glycosylase and apurinic/apyrimidinic (AP) site lyase. The former acts to excise damaged purine bases in dsDNA, creating an AP site; the latter can cleave at the 3′ and 5′ ends of an AP site, generating a gapped DNA molecule with 3′ and 5′ phosphate termini. The damaged bases primarily recognized by Fpg include: 7,8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, formamidopyrimidine (fapy) guanine, methyl-fapy-guanine, fapy adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil.

T4 Polynucleotide Kinase (RK20524)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5´-phosphates to oligonucleotides to allow subsequent ligation and removal of 3´-phosphoryl groups.