- Plastiki laboratoryjne
- NGS
- Zestawy DNASeq
- NGS DNA Library Prep Kit
- PCR-Free NGS DNA Library Prep Kit
- NGS Low Input DNA Library Prep Kit
- ChIP-Seq Library Prep Kit
- NGS Cell-free DNA Library Prep Kit
- NGS FFPE DNA Library Prep Kit
- Bisulfite Sequencing Library Prep Kit
- Methylation Specific Bisulfite-Seq Library Prep Kit
- NGS Single Stranded DNA Library Prep Kit
- NGS Ancient DNA Library Prep Kit
- NGS DNA Fragmentation & Library Prep Kit
- Zestawy RNASeq
- FFPE Transcriptomics
- Whole Transcriptome Library Prep Kits
- RNA Enrichment and Depletion
- Expression Profiling Library Prep Kits
- Small RNA Profiling & Discovery
- Single-cell and Low-input RNA-Seq Library Prep Kits
- Metabolic RNA Labeling
- Lexogen Indexing Solutions
- Spike-In RNA Controls
- RNA Stabilization / Extraction / Isolation
- cDNA Amplification
- Modules and Add-ons
- NGS Data Analysis Software
- Sekwencjonownie długich fragmentów
- Library Quantification kits
- Lexogen RNA-Seq, DNA-Seq – usługa
- DNA/RNA Purification (Magnetic Beads)
- Library Size Selection (Magnetic Beads)
- DNA Size Selection (Magnetic Beads)
- Single-cell RNA-Seq
- LUTHOR High-Definition Single-Cell 3’ mRNA-Seq
- LUTHOR single cell dispenser
- SeekGene Instruments / Reagents / Software
- SeekOne® Digital Droplet System
- SeekOne® DD Single Cell Full-length RNA Sequence Transcriptome-seq Kit
- SeekOne® DD Single Cell 3’transcriptome-seq Kit
- SeekOne® DD Single Cell Immune Profiling Kits
- SeekOne DD FFPE Single Cell RNA-seq Kit
- SeakSoul Tools – Single-cell Analysis Software
- SeekMate Tinitan® Fluorescence Cell Counter
- Zestawy DNASeq
- Biobankowanie
- 2D barcoded tubes
- Internal thread tubes – 96 SBS rack
- External thread tubes – 96 SBS rack
- Screw cap carrier – 96 SBS format
- Septum cap tubes – 96 SBS rack
- Septum cap mats – 96 SBS format
- Internal thread tubes – 48 SBS rack
- External thread tubes – 48 SBS rack
- External thread tubes – 24 SBS rack
- Racks – SBS format
- Racks – 14×14
- Internal thread tubes – Large rack
- External thread tubes – Large rack
- Racks – Large format
- 2D barcode readers
- Tube decapping
- MagCap™
- Lab tools
- 2D barcoded tubes
- PCR
- CRISPR
- Hodowle komórkowe
- Kryminalistyka
- Wymazówki Copan
- Zestawy STR
- Zestawy do identyfikacji śladów RSID
- Zestawy do barwienia immunofluorescencyjnego
- Zestawy do oczyszczania
- STK Sperm Tracker
- Phadebas Forensic Saliva Test
- Lumiscene
- Oświetlenie kryminalistyczne
- Okulary i oświetlacze kryminalistyczne
- Specjalistyczny sprzęt do oględzin
- Daktyloskopia
- Pozostałe
- Sprzęt
DNA Polymerase I (E. coli) (RK20530)
DNA Polymerase I (E coli) is a DNA-dependent DNA polymerase with inherent 3´→ 5´ and 5´→ 3´ exonuclease activities. The 5´→ 3´ exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation.
It is applicable to nick translation of DNA for obtaining probes with a high specific activity and for second strand synthesis of cDNA.
DNA Polymerase I, Large (Klenow) Fragment (RK20525)
DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3’→5′ exonuclease activity, but has lost 5’→3′ exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5′ termini.
It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5′ overhangs to form blunt ends, removal of 3′ overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.
E. coli Poly(A) Polymerase (RK20591)
Poly(A) polymerase catalyzes the addition of AMP, converted from ATP, to the 3′ end of RNA in a template-independent manner.
Klenow Fragment 3’→5′ exo- (RK20526)
Klenow Fragment (3’→ 5′ exo–) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5’→ 3′ exonuclease activity and has mutations (D355A, E357A) which abolish the 3’→ 5′ exonuclease activity. Klenow Fragment (3’→ 5′ exo–) is isolated from a recombinant source. It generates probes using random primers and shows moderate strand displacement activity. It can be used in random primer labeling, DNA sequencing by the Sanger dideoxy method, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.
T4 DNA Polymerase (5,000 U/mL) (RK20539)
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5’→3′ direction and requires the presence of template and primer. This enzyme has a 3’→5′ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5’→3′ exonuclease function.
It is applicable to 3′ overhang removal to form blunt ends, 5′ overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.
