Wyświetlanie wszystkich wyników: 10

Bsu DNA Polymerase (RK20566)

Bsu DNA Polymerase, Large Fragment retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity. Bsu DNA polymerase has strand displacement activity and is commonly used in recombinase polymerase amplification (RPA).

E.coli SSB (RK20590)

E. coli SSB (single-stranded binding) protein is a single-stranded DNA (ssDNA) binding protein essential for E. coli DNA replication and repair. The tetramer formed by the SSB protein specifically binds 8-16 nucleotides, thereby protecting ssDNA from nuclease degradation. In vitro experiments have shown that this protein can stabilize single-stranded regions of DNA, thereby relaxing double-stranded regions, which enhances the activity of DNA polymerase.

phi29 DNA Polymerase (RK21002)

phi29 DNA Polymerase is a replicative polymerase from the Bacillus subtilis phage phi29. phi29 DNA Polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5′ proofreading exonuclease activity. phi29 DNA Polymerase has advantages in replication requiring a high degree of strand displacement and/or processive synthesis, as well as high fidelity replication at moderate temperature.

Pilot phi29 DNA Polymerase (RK21006)

Pilot phi29 DNA Polymerase differs from Phi29 DNA Polymerase by significantly enhancing protein thermal stability, reaction speed, and salt tolerance while retaining all the advantages of the latter. Pilot phi29 DNA Polymerase exhibits strong multiple strand displacement, continuous synthesis characteristics, and 3 ‘-5’ exonuclease activity.

Ruby Bst DNA Polymerase (RK21005)

Ruby Bst DNA Polymerase is an in silico designed homologue of Geobacillus stearothermophilus DNA Polymerase I(large fragment). Ruby Bst DNA Polymerase has 5´→3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→3´ exonuclease activity. Ruby Bst DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermo stability compared with wild-type Bst DNA Polymerase, Large Fragment.

T4 Gene 32 Protein (10 mg/mL) (RK20546)

T4 Gene 32 Protein (10 mg/mL), also known as T4 gp32 protein, is a single stranded specific DNA binding protein. The native Gene 32 Protein from bacteriophage T4 (T4gp32) is a single-stranded DNA binding protein that is required for T4 DNA replication, recombination and repair. T4 Gene 32 protein can increase the yield of long PCR products when the reaction contains proteins ranging from 0.5 to 1.0 nmol. The T4 Gene 32 protein can increase the production of PCR products,reducing the inhibitor influence of humic acid. Meanwhile, the protein can promote the digestion reaction of restriction endonucleases, reverse transcription efficiency in RT-PCR, and enhance the activity of T4 DNA polymerase.

T4 UvsX Recombinase (RK20567)

T4 UvsX Recombinase is homologous to the RecA/Rad51 family. The RecA/Rad51 family of recombinases plays an important role in gene recombination, DNA repair, and replication, with pairing and strand replacement activities. T4 UvsX Recombinase can bind with primers to form a complex to search for homologous sequences in double stranded DNA and perform strand displacement reactions. It can work together with T4 UvsY protein, single stranded DNA binding protein, Bsu polymerase, etc. to complete isothermal amplification (RPA) of recombinase polymerase.

T4 UvsY Protein (RK20568)

T4 UvsY Protein can help T4 UvsX Recombinase compete for primers that pre bind to single stranded DNA binding proteins. It promotes the binding of T4 UvsX Recombinase with primers to form a complex, searching for homologous sequences in double stranded DNA for strand displacement reactions. It is mainly used in recombinase polymerase amplification (RPA).

Taq DNA Polymerase (Mg2+ Plus Buffer) (RK20600)

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity. Taq DNA Polymerase was isolated from a recombinant source and offers robust and reliable reactions. It tolerates a wide range of templates and incorporates dUTP, dITP, and fluorescently-labeled nucleotides.

Thermostable SSB (RK20589)

Thermostable SSB (Thermostable Single Stranded Binding Protein) is a single-stranded DNA-binding protein isolated from extremely heat-tolerant microorganisms. It remains fully activity after 60 min of incubation at 95°C. Due to its high thermal stability, Thermostable SSB can be used in experiments that require high-temperature reaction conditions, such as to increase PCR yield and specificity, as well as to increase isothermal amplification yields and enhance the efficiency of reverse transcription in RT-PCR.