ABScript III RT Master Mix for qPCR is developed based on ABScript II Reverse Transcriptase and suitable for two-step RT-qPCR detection. 5X ABScript III RT Mix contains all the reagents needed for reverse transcription reaction (ABScript III Reverse Transcriptase, RNase Inhibitor, RandomPrimer, Oligo dT Primer, dNTP and reaction buffer), and a reaction can be started simply by adding template RNA and Nuclease-free H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript III RT Master Mix for qPCR with gDNA Remover is developed based on ABScript III Reverse Transcriptase and suitable for two-step RT-qPCR detection. The 5X ABScript III RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance geneexpression analysis.