ABscript II Reverse transcriptase is a recombinant M-MuLV reverse transcriptase with low RNase H activity and enhanced thermal stability.Compared to wild-type M-MuLV,recombinant M-MuLV reverse transcriptase can synthesize first-strand cDNA at higher temperatures.The enzyme is active at up to 48℃ with higher specificity and cDNA yield.The kit provides two reverse transcription primers.The Oligo-dT primer [d(T)₂₃VN] forces the primer to anneal to the beginning of the polyA tail.The optimized random primer premix has low specificity,and all RNA,including mRNA,rRNA,and tRNA can be used as a reverse transcription template.This kit can amplify cDNA up to 10 kb.