High-Definition single-cell RNA sequencing
/ haɪ ˌdɛfɪˈnɪʃᵊn ˈsɪŋɡᵊl sɛl ɑːr-ɛn-eɪ ˈsiːkwənsɪŋ/

A single cell typically expresses thousands of different genes at the same time. Most of these genes are represented only in a few copies. High-Definition single-cell RNA sequencing (HD scRNA-Seq) collects any mRNA present in the sample, even those lowly expressed transcripts (less than 10 copies). HD scRNA-Seq, therefore, provides a comprehensive overview of the full transcriptomic status in each cell.

HD scRNA-Seq applications

High-Definition single-cell RNA sequencing works best with ultra-low initial inputs (as low as 10 pg RNA). By seeing more genes than classical bulk scRNA-Seq methods, it expands transcriptomic analysis into new scientific applications.

  •  High-definition single-cell differential gene expression analysis
  •  Detailed characterization of cell subpopulations after larger, less sensitive screenings (bulk scRNA-Seq)
  •  Rare cell RNA analysis (e.g., circulating tumor cells (CTCs) or innate lymphoid cells (ILCs))
  •  Subcellular (cytoplasm, organelle) RNA analysis
  •  Single nucleus RNA-Seq

Meet the LUTHOR family

LUTHOR solutions, developed by Lexogen, equip researchers with new tools to dive into single-cell RNA sequencing with unprecedented sensitivity. Lexogen’s LUTHOR portfolio currently includes:

Benefits

LUTHOR HD reaction allows to amplify minuscule amounts of RNA material and to obtain a full understanding of each cell’s gene expression status.

Unmatched sensitivity

See even low expressed genes (< 10 copies/cell)!

UMI included

Avoid any read count duplicates.

Direct RNA amplification

No more gDNA in your reads!

Compatible with "less-than-a-cell" inputs

Go as low as 1 pg!