LUTHOR High-Definition Single-Cell 3’ mRNA-Seq
High-Definition single-cell RNA sequencing
/ haɪ ˌdɛfɪˈnɪʃᵊn ˈsɪŋɡᵊl sɛl ɑːr-ɛn-eɪ ˈsiːkwənsɪŋ/
A single cell typically expresses thousands of different genes at the same time. Most of these genes are represented only in a few copies. High-Definition single-cell RNA sequencing (HD scRNA-Seq) collects any mRNA present in the sample, even those lowly expressed transcripts (less than 10 copies). HD scRNA-Seq, therefore, provides a comprehensive overview of the full transcriptomic status in each cell.
HD scRNA-Seq applications
High-Definition single-cell RNA sequencing works best with ultra-low initial inputs (as low as 10 pg RNA). By seeing more genes than classical bulk scRNA-Seq methods, it expands transcriptomic analysis into new scientific applications.
- High-definition single-cell differential gene expression analysis
- Detailed characterization of cell subpopulations after larger, less sensitive screenings (bulk scRNA-Seq)
- Rare cell RNA analysis (e.g., circulating tumor cells (CTCs) or innate lymphoid cells (ILCs))
- Subcellular (cytoplasm, organelle) RNA analysis
- Single nucleus RNA-Seq
Meet the LUTHOR family
LUTHOR solutions, developed by Lexogen, equip researchers with new tools to dive into single-cell RNA sequencing with unprecedented sensitivity. Lexogen’s LUTHOR portfolio currently includes:
- LUTHOR High-Definition Single-Cell 3’ mRNA-Seq Library Prep Kit
- LUTHOR High-Definition Pool Single-Cell 3’ mRNA-Seq Library Prep Kit
- LUTHOR single cell dispenser (with a unique validated workflow, from cell to library)
Benefits
LUTHOR HD reaction allows to amplify minuscule amounts of RNA material and to obtain a full understanding of each cell’s gene expression status.
Unmatched sensitivity
See even low expressed genes (< 10 copies/cell)!
UMI included
Avoid any read count duplicates.
Direct RNA amplification
No more gDNA in your reads!
