ABScript II cDNA First-Strand Synthesis Kit (RK20400)
ABscript II Reverse transcriptase is a recombinant M-MuLV reverse transcriptase with low RNase H activity and enhanced thermal stability.Compared to wild-type M-MuLV,recombinant M-MuLV reverse transcriptase can synthesize first-strand cDNA at higher temperatures.The enzyme is active at up to 48℃ with higher specificity and cDNA yield.The kit provides two reverse transcription primers.The Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail.The optimized random primer premix has low specificity,and all RNA,including mRNA,rRNA,and tRNA can be used as a reverse transcription template.This kit can amplify cDNA up to 10 kb.
Opis
Product Features
- The low activity of RNase H is beneficial to the synthesis of long fragment cDNA, which can synthesize cDNA up to 10 KB.
- The optimum temperature of the enzyme was 42℃ and the enzyme still has high activity at 48℃.
- The specific cDNA yield was higher than that of ordinary M-MLV reverse transcriptase (37℃).
- Suitable for coronavirus detection;
- With a variety of RT primers, both mRNA and ncRNA can be used as reverse transcription templates.
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