FFPE scRNA-seq
Random-primed single-nucleus RNA profiling optimized for FFPE samples.
Introduction
Highlights
Dual De-crosslinking Strategy
Improves RNA accessibility in FFPE samples
TSO-Extended Reverse Transcription
Enables longer RNA sequence coverage
Efficient Capture of Degraded RNA
High recovery from heavily crosslinked material
Expanded Priming Strategy
Oligo(dT) and random priming support diverse RNA species
Workflow
From Sample to Insight in Six Steps
Sample Prep
Nuclei isolation & RNA QC
In-situ Reverse Transcription
Catch RNA with Random Primer
Microfluidic Partitioning
Automated separation and labeling cells and RNA
Library Prep
Construct expression libraries
Sequencing
Generate datasets
Bioinformatic Analysis
Data processing and visualization
Product Features
- Human
- Rodents
- Primates
- Amphibians
- FFPE sample
Components
|
Product Name |
Product Code |
Specification |
|---|---|---|
|
SeekOne™ DD FFPE Single Cell Transcriptome-seq Kit |
K02101-08 |
8 tests |
|
Components Name |
Components Code |
Specification |
|---|---|---|
|
SeekOne™ DD Chip S3 Kit |
K00202-0801 |
8 tests |
|
SeekOne™ DD scFAST-seq Barcoded Beads Kit |
K00801-0802 |
8 tests |
|
SeekOne™ DD scFAST-seq Reverse Transcription Kit |
K00801-0803 |
8 tests |
|
SeekOne™ DD Single Cell Barcode Ligation Kit |
K02101-0804 |
8 tests |
|
SeekOne™ DD Single Cell Decrosslinking Kit |
K02101-0806 |
8 tests |
|
SeekOne™ DD Single Cell Library Amplification Kit |
K02101-0807 |
8 tests |
|
SeekOne™ DD Single Cell Cleanup Kit |
K00202-0805 |
8 tests |
Compatible Instrument
SeekOne™ Digital Droplet System
A high-throughput, automated single-cell multi-omics platform based on microfluidic water-in-oil technology for single cell/nucleus separation and barcoding.
Compatible Software
SeekSoul™ Tools
SeekSoul™ Online
Data Analysis
SeekSoul™ Tools
SeekSoul™ Online
