—— Investigate gene expression with accuracy and efficiency

SeekOne DD (Digital Droplet) Single Cell 3′ Transcriptome-seq is a high-throughput single-cell RNA-seq library kit achieves single-cell partitioning and labelling by the microfluidic system and barcoded beads. The kit needs to be used with the SeekOne Digital Droplet System (SeekOne DD) to complete the whole process from single-cell transcriptome barcoding to library construction.

SeekOne DD single cell 3′ transcriptome kit includes: SeekOne DD Chip S3 (Chip S3 for short), Gasket, Carrier Oil, SeekOneDD 3′ Barcoded Beads (Barcoded Beads for short), amplification reagents, library construction reagents, and single cell data analysis software (SeekSoulTools). Based on the principle of microfluidic technology, the kit uses emulsion droplets to separate and capture individual cells, and then recruits nucleic acid-modified Barcoded Beads to molecularly label RNA from different cell sources. Finally, a high-throughput single-cell transcriptome library compatible with Illumina and MGI sequencing system will be constructed, which can be used for research on tumors, immunity, cell development, viral infections, drug discovery, and target screening, etc.

Workflow

1. Single cell capture and labelling

Cells and barcoded beads are added separately and then react in the carrier oil to form emulsion droplets in the –shaped channel. After that, mRNA molecules released by the cells are captured by oligo(dT) on the barcoded beads.

1-8 samples can be flexibly run at a time

500-12,000 cells can be captured with a single channel 

150,000 emulsion droplets can be generated in 3 minutes

High cell capture rates of up to 65%

Recovery of ~1000 cells with doublet rates of ~0.3% per 1,000 cells

2. Library Construction

Product Features

Compatible

Compatible with single cell 3' transcriptome sequencing (3' scRNA-seq) and single nucleus RNA sequencing (snRNA-seq). Dual index libraries compatible with both Illumina and MGI sequencers.

Fast

Rapid generation of 150,000 water-in-oil droplets in 3 minutes with high success rates, especially for precious and sensitive samples.

Cost-saving

nique place chip design eliminates chip waste, reducing operation time and cost

Suitable sample types

a. Delicate samples with low cell viability:brain tissue, retinal tissue, all tissues from aged individuals
b. Tissues requiring nuclei isolation: frozen tissues, pancreatic tissues, neurons, adipose tissue, etc.
c. Large cohort or atlas studies where multiple samples need to be processed simultaneously.