—— The Time Has Come for A New Generation of Single Cell Transcriptome Profiling

SeekOne Digital Droplet (SeekOne DD) High throughput Single Cell Full-length RNA Sequence Transcriptome-seq (scFAST-seq) Kit, self- developed by Beijing SeekGene BioSciences Co., Ltd., is a powerful commercial tool for high-throughput whole transcriptome profiling. Compared to conventional 3′ scRNA-seq, scFAST- seq has distinct advantages in detecting non- polyadenylated transcripts, transcript coverage length, and identification of more splice junctions. With target region enrichment, scFAST-seq can simultaneously detect somatic mutations and cell states in individual tumour cells, providing valuable information for precision medicine.

SeekOne DD Single Cell Full-length RNA Sequence Transcriptome-seq (scFAST-seq) Kit includes SeekOne DD Chip S3 (Chip S3), Gasket, Carrier Oil, SeekOne DD scFAST-seq Barcoded Beads, amplification reagents, library construction reagents.

Core technology and workflow

Using microfluidic digital droplets and Barcoded Beads coupled with random primers, single-cell partition and random capture of the whole transcriptome can be achieved in a step of generating water-in-oil droplets. This convenient process requires only a small amount of sample. The inclusion of an rRNA blocker enables efficient depletion of rRNA, delivering higher proportion of effective data

1-8 samples can be flexibly run at a time

500-12,000 cells can be captured with a single channel 

150,000 emulsion droplets can be generated in 3 minutes

Recovery of ~1000 cells with doublet rates of ~0.3% per 1,000 cells

Even coverage of the full-length of the gene from 5′ to 3’end

Flexibility to incorporate a panel for detecting target genes with high

Product Features

Accurate single-cell transcriptome

Achieve full-length transcriptome detection without being limited by the 3' or 5' end.

Genotype to phenotype

Simultaneously obtain single-cell mutation and gene expression information, establishing the relationship between genotype and phenotype.

Capturing non-coding RNA

In addition to mRNA, non-coding RNA without 3' polyA tails can also be captured.

Crossing the species barrier

Enable the detection of viruses and prokaryotes.

Improving analysis accuracy

Allow the detection of isoform switching events of mRNA and IncRNA.

Achieving effective rRNA depletion

the inclusion of an rRNA blocker enables efficient depletion of rRNA,

Applications

Compared to conventional 3′ scRNA-seq, scFAST- seq has distinct advantages in detecting non- polyadenylated transcripts, transcript coverage length, and identification of more splice junctions. With target region enrichment, scFAST-seq can simultaneously detect somatic mutations and cell states in individual tumour cells, providing valuable information for precision medicine.

  • Detection of single cell mutation
  • Detection of more splice junctions
  • Identification of non-polyA tailed viral infected cell clusters
  • Clearer and more accurate cell trajectory inference compared to the 3' platform
  • scFAST-seq data can be integrated with 3' data