- Plastiki laboratoryjne
- NGS
- Zestawy DNASeq
- NGS DNA Library Prep Kit
- PCR-Free NGS DNA Library Prep Kit
- NGS Low Input DNA Library Prep Kit
- ChIP-Seq Library Prep Kit
- NGS Cell-free DNA Library Prep Kit
- NGS FFPE DNA Library Prep Kit
- Bisulfite Sequencing Library Prep Kit
- Methylation Specific Bisulfite-Seq Library Prep Kit
- NGS Single Stranded DNA Library Prep Kit
- NGS Ancient DNA Library Prep Kit
- NGS DNA Fragmentation & Library Prep Kit
- Zestawy RNASeq
- FFPE Transcriptomics
- Whole Transcriptome Library Prep Kits
- RNA Enrichment and Depletion
- Expression Profiling Library Prep Kits
- Small RNA Profiling & Discovery
- Single-cell and Low-input RNA-Seq Library Prep Kits
- Metabolic RNA Labeling
- Lexogen Indexing Solutions
- Spike-In RNA Controls
- RNA Stabilization / Extraction / Isolation
- cDNA Amplification
- Modules and Add-ons
- NGS Data Analysis Software
- Sekwencjonownie długich fragmentów
- Library Quantification kits
- Lexogen RNA-Seq, DNA-Seq – usługa
- DNA/RNA Purification (Magnetic Beads)
- Library Size Selection (Magnetic Beads)
- DNA Size Selection (Magnetic Beads)
- Single-cell RNA-Seq
- LUTHOR High-Definition Single-Cell 3’ mRNA-Seq
- LUTHOR single cell dispenser
- SeekGene Instruments / Reagents / Software
- SeekOne® Digital Droplet System
- SeekOne® DD Single Cell Full-length RNA Sequence Transcriptome-seq Kit
- SeekOne® DD Single Cell 3’transcriptome-seq Kit
- SeekOne® DD Single Cell Immune Profiling Kits
- SeekOne DD FFPE Single Cell RNA-seq Kit
- SeakSoul Tools – Single-cell Analysis Software
- SeekMate Tinitan® Fluorescence Cell Counter
- Zestawy DNASeq
- Biobankowanie
- 2D barcoded tubes
- Internal thread tubes – 96 SBS rack
- External thread tubes – 96 SBS rack
- Screw cap carrier – 96 SBS format
- Septum cap tubes – 96 SBS rack
- Septum cap mats – 96 SBS format
- Internal thread tubes – 48 SBS rack
- External thread tubes – 48 SBS rack
- External thread tubes – 24 SBS rack
- Racks – SBS format
- Racks – 14×14
- Internal thread tubes – Large rack
- External thread tubes – Large rack
- Racks – Large format
- 2D barcode readers
- Tube decapping
- MagCap™
- Lab tools
- 2D barcoded tubes
- PCR
- CRISPR
- Hodowle komórkowe
- Kryminalistyka
- Wymazówki Copan
- Zestawy STR
- Zestawy do identyfikacji śladów RSID
- Zestawy do barwienia immunofluorescencyjnego
- Zestawy do oczyszczania
- STK Sperm Tracker
- Phadebas Forensic Saliva Test
- Lumiscene
- Oświetlenie kryminalistyczne
- Okulary i oświetlacze kryminalistyczne
- Specjalistyczny sprzęt do oględzin
- Daktyloskopia
- Pozostałe
- Sprzęt
SeekOne® DD Single Cell Full-length RNA Sequence Transcriptome-seq Kit
—— The Time Has Come for A New Generation of Single Cell Transcriptome Profiling
SeekOne Digital Droplet (SeekOne DD) High throughput Single Cell Full-length RNA Sequence Transcriptome-seq (scFAST-seq) Kit, self- developed by Beijing SeekGene BioSciences Co., Ltd., is a powerful commercial tool for high-throughput whole transcriptome profiling. Compared to conventional 3′ scRNA-seq, scFAST- seq has distinct advantages in detecting non- polyadenylated transcripts, transcript coverage length, and identification of more splice junctions. With target region enrichment, scFAST-seq can simultaneously detect somatic mutations and cell states in individual tumour cells, providing valuable information for precision medicine.
SeekOne DD Single Cell Full-length RNA Sequence Transcriptome-seq (scFAST-seq) Kit includes SeekOne DD Chip S3 (Chip S3), Gasket, Carrier Oil, SeekOne DD scFAST-seq Barcoded Beads, amplification reagents, library construction reagents.
Core technology and workflow
Using microfluidic digital droplets and Barcoded Beads coupled with random primers, single-cell partition and random capture of the whole transcriptome can be achieved in a step of generating water-in-oil droplets. This convenient process requires only a small amount of sample. The inclusion of an rRNA blocker enables efficient depletion of rRNA, delivering higher proportion of effective data

Recovery of ~1000 cells with doublet rates of ~0.3% per 1,000 cells
Even coverage of the full-length of the gene from 5′ to 3’end
Flexibility to incorporate a panel for detecting target genes with high
Product Features

Accurate single-cell transcriptome
Achieve full-length transcriptome detection without being limited by the 3' or 5' end.

Genotype to phenotype
Simultaneously obtain single-cell mutation and gene expression information, establishing the relationship between genotype and phenotype.

Capturing non-coding RNA
In addition to mRNA, non-coding RNA without 3' polyA tails can also be captured.

Crossing the species barrier
Enable the detection of viruses and prokaryotes.

Improving analysis accuracy
Allow the detection of isoform switching events of mRNA and IncRNA.

Achieving effective rRNA depletion
the inclusion of an rRNA blocker enables efficient depletion of rRNA,
Applications
Compared to conventional 3′ scRNA-seq, scFAST- seq has distinct advantages in detecting non- polyadenylated transcripts, transcript coverage length, and identification of more splice junctions. With target region enrichment, scFAST-seq can simultaneously detect somatic mutations and cell states in individual tumour cells, providing valuable information for precision medicine.
- Detection of single cell mutation

- Detection of more splice junctions

- Identification of non-polyA tailed viral infected cell clusters

- Clearer and more accurate cell trajectory inference compared to the 3' platform

- scFAST-seq data can be integrated with 3' data
