—— Reveal the immune specificity with complete V(D)J sequences

SeekOne DD single-cell immune profiling (5′ transcriptome & immune repertoire sequencing), proposed by Beijing SeekGene BioSciences Co., Ltd., achieves single-cell partitioning and labelling by the microfluidic system and barcoded beads. The library of both full-length TCR/BCR sequences and mRNA sequences can be constructed from a single sample, followed by high-throughput sequencing and bioinformatics analysis.

The SeekOne DD Single Cell Immune Profiling Kits include the SeekOne DD Single Cell 5’ Transcriptome-seq Kit and the SeekOneDD TCR/BCR amplification Kit. With this kits, the full-length sequence of V(D)J, the pairing information of α chain (heavy chain) and β chain (light chain) of TCR(BCR) and the transcriptome information can be obtained simultaneously. It can be used to investigate T/B cell heterogeneity, TCR/BCR diversity, antigen specificity, vaccine development, immunetherapy screening, etc.

Core technology and workflow

1.Single cell capture and labelling

SeekOne DD single cell immune profiling products are based on the principle of microfluidic technology. Cells and barcoded beads are added separately and then react in the carrier oil to form emulsion droplets in the – shaped channel. After capturing mRNA tagged with barcoded beads, two separate libraries (gene expression & immune repertoire) can be constructed from a single sample.

  • Up to 90% of reads mapped to any V(D)J gene
  • Rapid generation of 150000 water-in-oil droplets in 3 minutes
  • Efficiently capture 500-12,000 cells per channel
  • Flexible running of 1~8 samples in parallel
  • High cell capture rates of up to 65%
  • Low doublet rates of under 0.3% per 1,000 cells

2. Library Construction

Product Features

Full length

Complete coverage of the full-length V(D)J sequences. Analyze all V(D)J sequences, including CDR3 sequences, for more comprehensive information

Efficient capture

Efficiently capture V(D)J sequences with an alignment rate greater than 90%. Obtain more accurate and authentic capture data

True alignment

True representation of α/β (light/heavy) chain pairing information of TCR (BCR). Reports pairing information from the same cell to reveal receptor-antigen specificity of the immune cells

One sample with two profiles

Simultaneous profiling of immune repertoire (BCR/TCR) and 5’ gene expression Multiple libraries are constructed from a single sample, which is more economical and suitable for samples with limited amounts, such as needle biopsy samples.