- Plastiki laboratoryjne
- NGS
- Zestawy DNASeq
- NGS DNA Library Prep Kit
- PCR-Free NGS DNA Library Prep Kit
- NGS Low Input DNA Library Prep Kit
- ChIP-Seq Library Prep Kit
- NGS Cell-free DNA Library Prep Kit
- NGS FFPE DNA Library Prep Kit
- Bisulfite Sequencing Library Prep Kit
- Methylation Specific Bisulfite-Seq Library Prep Kit
- NGS Single Stranded DNA Library Prep Kit
- NGS Ancient DNA Library Prep Kit
- NGS DNA Fragmentation & Library Prep Kit
- Zestawy RNASeq
- FFPE Transcriptomics
- Whole Transcriptome Library Prep Kits
- RNA Enrichment and Depletion
- Expression Profiling Library Prep Kits
- Small RNA Profiling & Discovery
- Single-cell and Low-input RNA-Seq Library Prep Kits
- Metabolic RNA Labeling
- Lexogen Indexing Solutions
- Spike-In RNA Controls
- RNA Stabilization / Extraction / Isolation
- cDNA Amplification
- Modules and Add-ons
- NGS Data Analysis Software
- Sekwencjonownie długich fragmentów
- Library Quantification kits
- Lexogen RNA-Seq, DNA-Seq – usługa
- DNA/RNA Purification (Magnetic Beads)
- Library Size Selection (Magnetic Beads)
- DNA Size Selection (Magnetic Beads)
- Single-cell RNA-Seq
- LUTHOR High-Definition Single-Cell 3’ mRNA-Seq
- LUTHOR single cell dispenser
- SeekGene Instruments / Reagents / Software
- SeekOne® Digital Droplet System
- SeekOne® DD Single Cell Full-length RNA Sequence Transcriptome-seq Kit
- SeekOne® DD Single Cell 3’transcriptome-seq Kit
- SeekOne® DD Single Cell Immune Profiling Kits
- SeekOne DD FFPE Single Cell RNA-seq Kit
- SeakSoul Tools – Single-cell Analysis Software
- SeekMate Tinitan® Fluorescence Cell Counter
- Zestawy DNASeq
- Biobankowanie
- 2D barcoded tubes
- Internal thread tubes – 96 SBS rack
- External thread tubes – 96 SBS rack
- Screw cap carrier – 96 SBS format
- Septum cap tubes – 96 SBS rack
- Septum cap mats – 96 SBS format
- Internal thread tubes – 48 SBS rack
- External thread tubes – 48 SBS rack
- External thread tubes – 24 SBS rack
- Racks – SBS format
- Racks – 14×14
- Internal thread tubes – Large rack
- External thread tubes – Large rack
- Racks – Large format
- 2D barcode readers
- Tube decapping
- MagCap™
- Lab tools
- 2D barcoded tubes
- PCR
- CRISPR
- Hodowle komórkowe
- Kryminalistyka
- Wymazówki Copan
- Zestawy STR
- Zestawy do identyfikacji śladów RSID
- Zestawy do barwienia immunofluorescencyjnego
- Zestawy do oczyszczania
- STK Sperm Tracker
- Phadebas Forensic Saliva Test
- Lumiscene
- Oświetlenie kryminalistyczne
- Okulary i oświetlacze kryminalistyczne
- Specjalistyczny sprzęt do oględzin
- Daktyloskopia
- Pozostałe
- Sprzęt
SeekOne® DD Single Cell Immune Profiling Kits
—— Reveal the immune specificity with complete V(D)J sequences
SeekOne DD single-cell immune profiling (5′ transcriptome & immune repertoire sequencing), proposed by Beijing SeekGene BioSciences Co., Ltd., achieves single-cell partitioning and labelling by the microfluidic system and barcoded beads. The library of both full-length TCR/BCR sequences and mRNA sequences can be constructed from a single sample, followed by high-throughput sequencing and bioinformatics analysis.
The SeekOne DD Single Cell Immune Profiling Kits include the SeekOne DD Single Cell 5’ Transcriptome-seq Kit and the SeekOneDD TCR/BCR amplification Kit. With this kits, the full-length sequence of V(D)J, the pairing information of α chain (heavy chain) and β chain (light chain) of TCR(BCR) and the transcriptome information can be obtained simultaneously. It can be used to investigate T/B cell heterogeneity, TCR/BCR diversity, antigen specificity, vaccine development, immunetherapy screening, etc.

Core technology and workflow
1.Single cell capture and labelling
SeekOne DD single cell immune profiling products are based on the principle of microfluidic technology. Cells and barcoded beads are added separately and then react in the carrier oil to form emulsion droplets in the – shaped channel. After capturing mRNA tagged with barcoded beads, two separate libraries (gene expression & immune repertoire) can be constructed from a single sample.

- Up to 90% of reads mapped to any V(D)J gene
- Rapid generation of 150000 water-in-oil droplets in 3 minutes
- Efficiently capture 500-12,000 cells per channel
- Flexible running of 1~8 samples in parallel
- High cell capture rates of up to 65%
- Low doublet rates of under 0.3% per 1,000 cells
2. Library Construction

Product Features

Full length
Complete coverage of the full-length V(D)J sequences. Analyze all V(D)J sequences, including CDR3 sequences, for more comprehensive information

Efficient capture
Efficiently capture V(D)J sequences with an alignment rate greater than 90%. Obtain more accurate and authentic capture data

True alignment
True representation of α/β (light/heavy) chain pairing information of TCR (BCR). Reports pairing information from the same cell to reveal receptor-antigen specificity of the immune cells

One sample with two profiles
Simultaneous profiling of immune repertoire (BCR/TCR) and 5’ gene expression Multiple libraries are constructed from a single sample, which is more economical and suitable for samples with limited amounts, such as needle biopsy samples.