—— Feel life with speed

SeekOneDigital Droplet System (SeekOneDD System), self-developed by Beijing SeekGene BioSciences Co., Ltd., achieves single-cell partitioning and capture by water-in-oil droplets generated in the microfluidic system. This provides a one-stop shop for single-cell solutions, from single-cell partitioning, trapping, labelling and library construction to data analysis. It can be applied to a variety of medical studies, including cancer, immunity, cell development, viral infection, drug discovery, and target screening.

Core technology

Cells and barcoded beads are added separately and then react in the carrier oil to form emulsion droplets in the –shaped channel. After that, mRNA molecules released by the cells are captured by oligo(dT) on the barcoded beads.

Workflow

The process begins with the collection of single cell suspension. After single cell partitioning, capture and labelling, a single cell library compatible with Illumina and MGI sequencers is constructed for high-throughput sequencing. Data can be processed using SeekSoul® Tools ——the efficient data analysis software to explore cell heterogeneity. 

Product Specifications

Product Features

Fast

Rapidly generates 150,000 water-in-oil droplets in 3 minutes, reducing cell loss.

Efficient

Up to 12,000 cells can be captured in a single channel. Flexible running of 1~8 samples in parallel.

Accurate

Low doublet rates of less than 0.3% per 1,000 cells; equipped with a temperature control system to eliminate the effects of environmental temperature differences.

Compatible

Highly compatible system with a real-time remote upgrade can be used with comprehensive SeekOneproduct lines to meet a variety of scientific research needs.

User-friendly

Small instrument with large screen; 7" + 45° view touch interface makes “one-click” operation more convenient and comfortable.

Cost-saving

Vacant channels can be occupied by Place Chip without wasting chips.

Accessories

Accessories, each instrument is equipped with 1 set of this accessory, including the following two parts:

SeekOne DD Chip Holder

Chip Holder, used with SeekOne® digital droplet system and supporting chips

Placed Chip

Placed Chip (Chip P for short), placed in a Chip Holder (8 Chip Ps are attached to each instrument); When the sample throughput is less than 8, Chip P is placed in the wells