The PCR Add-on kit contains a PCR Mix including Ion Torrent specific primer and a thermostable polymerase. With the PCR Add-on kit more endpoint PCRs can be run for your sample to determine the exact number of cycles needed to prevent any under- or overcycling of your library. Undercycling may result in too little library while overcycling can lead to significant distortions in gene expression values. If your sample is undercycled the kit can furthermore be used to add more cycles and reamplify your library.
•Kit size: 96 rxn
•Compatibility information ◦Product: QuantSeq 3’mRNA-Seq
◦Platform: Ion Torrent