With the QuantSeq-Flex Second Strand Synthesis Module V2 the random second strand synthesis primer can be substituted with a target-specific primer in the QuantSeq protocol. Please note that the second strand synthesis reaction requires annealing temperatures of >45 °C. In case you need lower temperatures, please contact info@lexogen.com.
•Kit size: 96 rxn
•Compatibility information ◦Product: QuantSeq 3’mRNA-Seq FWD
◦Platform: Illumina