LUTHOR High-Definition Single-Cell 3′ mRNA-Seq Kit with UDI 12 nt Set B1
LUTHOR HD redefines the approach to single-cell RNA sequencing by offering an RNA-amplification-based kit able to detect virtually every single mRNA molecule present in the cell.
Like QuantSeq FWD, LUTHOR HD is a 3′ end mRNA-Seq kit that uses oligo(dT) primers to target polyadenylated RNAs and generate reads mapping to the 3′ end of transcripts. The two kits, however, use different protocols and technologies and are developed for different sample input amounts. While LUTHOR HD is designed for inputs ranging from 1 ng to 10 pg (equivalent to 100 cells to 1 cell) and even lower*, QuantSeq FWD is ideal for inputs ranging from 1 ng to 500 ng of total RNA.
LUTHOR HD is compatible with Lexogen’s patented 12 nt UDI Sets for maximal read rescue capabilities (no more misassigned or lost reads!).
* We have pushed the boundaries of sensitivity even further. LUTHOR HD enables to work with inputs as low as 1 pg, e.g. with cytoplasmic extracts
LUTHOR High-Definition Single-Cell 3′ mRNA-Seq Kit without UDI 12 nt Set
LUTHOR HD redefines the approach to single-cell RNA sequencing by offering an RNA-amplification-based kit able to detect virtually every single mRNA molecule present in the cell.
Like QuantSeq FWD, LUTHOR HD is a 3′ end mRNA-Seq kit that uses oligo(dT) primers to target polyadenylated RNAs and generate reads mapping to the 3′ end of transcripts. The two kits, however, use different protocols and technologies and are developed for different sample input amounts. While LUTHOR HD is designed for inputs ranging from 1 ng to 10 pg (equivalent to 100 cells to 1 cell) and even lower*, QuantSeq FWD is ideal for inputs ranging from 1 ng to 500 ng of total RNA.
LUTHOR HD is compatible with Lexogen’s patented 12 nt UDI Sets for maximal read rescue capabilities (no more misassigned or lost reads!).
* We have pushed the boundaries of sensitivity even further. LUTHOR HD enables to work with inputs as low as 1 pg, e.g. with cytoplasmic extracts
For full flexibility, these LUTHOR kits are delivered without the required indices. We recommend the Lexogen UDI 12 nt Sets (Cat. No. 101 – 105)
QuDye dsDNA HS Assay kit (optimized for Qubit®) 100 assays
- optimized for Qubit® fluorometers featuring high sensitivity (HS)
- measurements range from 10 pg/uL to 100 ng/uL.
- kit includes the concentrated QuDye HS assay reagent, dilution buffer, and DNA standards (0 and 10 ng/uL)
QuDye dsDNA HS Assay kit (optimized for Qubit®) 500 assays
- optimized for Qubit® fluorometers featuring high sensitivity (HS)
- measurements range from 10 pg/uL to 100 ng/uL.
- kit includes the concentrated QuDye HS assay reagent, dilution buffer, and DNA standards (0 and 10 ng/uL)
Poly(A) RNA Selection Kit V1.5, 96 preps
The Poly(A) RNA Selection Module enables the rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.
Optimized V1.5 Upgrade
The upgrade V1.5 contains new oligo(dT) Magnetic Beads (MB), compared to the V1.0 kit. The beads and solutions included in the V1.5 kit offer improved performance.
Highly Specific for Poly(A) RNA
Other RNA species (rRNA and tRNA) do not contain poly(A) sequences and therefore will not bind to the oligo(dT) beads.
Various Downstream Applications
Isolated mRNA can directly be used for RNA-Seq library preparation (e.g., CORALL RNA-Seq Library Prep kits), SAGE, CAGE, cloning, microarrays, cDNA synthesis, and others.
Rapid Turnaround
Polyadenylated RNAs can be isolated from total RNA samples within about one hour.
QuDye dsDNA HS Assay kit (optimized for Qubit®) 100 assays (incl. tubes)
- optimized for Qubit® fluorometers featuring high sensitivity (HS)
- measurements range from 10 pg/uL to 100 ng/uL.
- kit includes the concentrated QuDye HS assay reagent, dilution buffer, DNA standards (0 and 10 ng/uL) and tubes
QuDye dsDNA HS Assay kit (optimized for Qubit®) 500 assays 500 (incl. tubes)
- optimized for Qubit® fluorometers featuring high sensitivity (HS)
- measurements range from 10 pg/uL to 100 ng/uL.
- kit includes the concentrated QuDye HS assay reagent, dilution buffer, DNA standards (0 and 10 ng/uL) and tubes
NGS Library Quantification Standards with PCR Primers (illumina)
Kit contents:
- Library Standards 1
- Library Standards 2
- Library Standards 3
- Library Standards 4
- Library Standards 5
- Library Standards 6
- Primer Mix
(80 ul X6 dilutions)
NGS Library Quantification Standards with PCR Primers (illumina)
Kit contents:
- Library Standards 1
- Library Standards 2
- Library Standards 3
- Library Standards 4
- Library Standards 5
- Library Standards 6
- Primer Mix
(320 ul X6 dilutions)
TELL-Seq™ WGS Library Prep Kit
Ceny dostępne dla zalogowanych UżytkownikówStandard pacage includes:
– Enzyme Mixes (box 1) – cat. No 100001
– TELL-Beads (box 2) – cat. No 100002
– Multiplex Primers (set 1-8) – cat. No 100003
– Illimina Primers – cat. No 100004
SureSelect users will also need a UST TELL-Seq™ Target Blocker 8-Rxn Kit
TELL-Seq™ WGS Library Prep Kit HT24
Ceny dostępne dla zalogowanych UżytkownikówHT package includes:
– Enzyme Mixes HT24 – cat. No 100011
– TELL-Beads HT24 – cat. No 100012
– Multiplex Primers (set 1-8) – cat. No 100003
– Illimina Primers HT 24- cat. No 100013
– Multiplex Primers (set 9-16) – cat. No 100009
– Multiplex Primers (set 17-24) – cat. No 100010
RiboCop rRNA Depletion Kit
Ceny dostępne dla zalogowanych UżytkownikówRiboCop rRNA Depletion Kits enable removal of ribosomal RNA from total RNA and are compatible with all random primed Library Prep Kits such as the CORALL RNA-Seq V2 Library Prep Kits (Cat. No. 171 – 176) for whole transcriptome sequencing.
RiboCop is available for various species:
- Human/Mouse/Rat (HMR, Cat. No. 144),
- Human/Mouse/Rat plus Globin (HMR+Globin, Cat. No. 145),
- Bacteria (Cat. No. 125 – 127), and
- Yeast (Cat. No. 190)
RiboCop kits are available as stand-alone kits in different sizes (24 and 96 preps).
For convenience, RiboCop for Human/Mouse/Rat (HMR or HMR+Globin) is also available as bundle with CORALL Total RNA-Seq Library Prep Kits. For optimal performance, we recommend CORALL Total RNA-Seq V2 Bundles with RiboCop rRNA depletion (Cat. No. 183 – 186).
RiboCop for Bacteria is available with three optimized Probe Mixes for depletion of rRNA from:
- mixed bacterial samples (META, Cat. No. 125),
- from Gram negative bacteria (G-, Cat. No. 126),
- or Gram positive bacteria (G+, Cat. No. 127).
New! RiboCop rRNA Depletion allows to unlock the complete yeast transcriptome and analyze non-coding RNAs.
Applications:
- Expression analysis, incl. non-coding transcripts
- Analysis of long non-coding RNA, snRNAs, snoRNAs, and circRNAs
- FFPE and Biobank transcriptomics
- Bacterial transcriptome research
- Metatranscriptomics
- Host-pathogen studies
- Yeast transcriptomics
Kit Content:
- Probe Mixes, Buffers, and Purification Beads
