- Plastiki laboratoryjne
- NGS
- Zestawy DNASeq
- NGS DNA Library Prep Kit
- PCR-Free NGS DNA Library Prep Kit
- NGS Low Input DNA Library Prep Kit
- ChIP-Seq Library Prep Kit
- NGS Cell-free DNA Library Prep Kit
- NGS FFPE DNA Library Prep Kit
- Bisulfite Sequencing Library Prep Kit
- Methylation Specific Bisulfite-Seq Library Prep Kit
- NGS Single Stranded DNA Library Prep Kit
- NGS Ancient DNA Library Prep Kit
- NGS DNA Fragmentation & Library Prep Kit
- Zestawy RNASeq
- FFPE Transcriptomics
- Whole Transcriptome Library Prep Kits
- RNA Enrichment and Depletion
- Expression Profiling Library Prep Kits
- Small RNA Profiling & Discovery
- Single-cell and Low-input RNA-Seq Library Prep Kits
- Metabolic RNA Labeling
- Lexogen Indexing Solutions
- Spike-In RNA Controls
- RNA Stabilization / Extraction / Isolation
- cDNA Amplification
- Modules and Add-ons
- NGS Data Analysis Software
- Sekwencjonownie długich fragmentów
- Library Quantification kits
- Lexogen RNA-Seq, DNA-Seq – usługa
- DNA/RNA Purification (Magnetic Beads)
- Library Size Selection (Magnetic Beads)
- DNA Size Selection (Magnetic Beads)
- Single-cell RNA-Seq
- LUTHOR High-Definition Single-Cell 3’ mRNA-Seq
- LUTHOR single cell dispenser
- SeekGene Instruments / Reagents / Software
- SeekOne® Digital Droplet System
- SeekOne® DD Single Cell Full-length RNA Sequence Transcriptome-seq Kit
- SeekOne® DD Single Cell 3’transcriptome-seq Kit
- SeekOne® DD Single Cell Immune Profiling Kits
- SeekOne DD FFPE Single Cell RNA-seq Kit
- SeakSoul Tools – Single-cell Analysis Software
- SeekMate Tinitan® Fluorescence Cell Counter
- Zestawy DNASeq
- Biobankowanie
- 2D barcoded tubes
- Internal thread tubes – 96 SBS rack
- External thread tubes – 96 SBS rack
- Screw cap carrier – 96 SBS format
- Septum cap tubes – 96 SBS rack
- Septum cap mats – 96 SBS format
- Internal thread tubes – 48 SBS rack
- External thread tubes – 48 SBS rack
- External thread tubes – 24 SBS rack
- Racks – SBS format
- Racks – 14×14
- Internal thread tubes – Large rack
- External thread tubes – Large rack
- Racks – Large format
- 2D barcode readers
- Tube decapping
- MagCap™
- Lab tools
- 2D barcoded tubes
- PCR
- CRISPR
- Hodowle komórkowe
- Kryminalistyka
- Wymazówki Copan
- Zestawy STR
- Zestawy do identyfikacji śladów RSID
- Zestawy do barwienia immunofluorescencyjnego
- Zestawy do oczyszczania
- STK Sperm Tracker
- Phadebas Forensic Saliva Test
- Lumiscene
- Oświetlenie kryminalistyczne
- Okulary i oświetlacze kryminalistyczne
- Specjalistyczny sprzęt do oględzin
- Daktyloskopia
- Pozostałe
- Sprzęt
cfDNA Purification Kit (Magnetic Beads)
The cfDNA Purification Kit (Magnetic Beads) was developed for cell free DNA (cfDNA) enrichment by separating genomic DNA contamination from isolated cfDNA samples.
Many diagnostic technologies for detection of disease signals in cfDNA begin with isolation and purification of DNA from liquid biopsy that include urine, plasma, cerebrospinal fluid. The most widely explored biotechnology is assays used to detect cancer-derived plasma cfDNA. Silica-based magnetic bead cfDNA isolation kits can reliably extract total DNA from plasma, but typically yield a large variation in cfDNA that includes the presence of genomic DNA that often depends on tumor stage, tumor size, or healthy status individuals. Most of the commercial cfDNA isolation kits can’t specifically recover the cfDNA while leaving the high molecular weight genomic DNA behind. The presence of genomic DNA can lead to decreased sensitivity or inconsistent results in downstream applications such as next-generation sequencing (NGS), PCR, QPCR, and digital PCR etc.
Therefore, an additional purification step to enrich cfDNA before downstream methods helps to improve signal from fragments that originate from cancer cells. A proportion of cancer-derived cfDNA fragment signals are below 100 bp and are often not detectable except by qPCR or single-stranded DNA based library preparation for NGS (1, 2, 3). Furthermore only 1% of cancer-derived fragments are found above 400 bp (1, 2). Capture of size-selected fragments between 90-150 bp improved detection of cancer by 2-4 fold (4). Furthermore, TF-bound and protected cfDNA fragments are also being investigated for active cancer-specific signals down to 35-80 bp (5, 6).
Custom Bioinformatics Solutions
With decades of experience in transcriptomics, genomics, and related fields of biology, our bioinformatics team is empowered to tailor new bioinformatics developments to your unique project. We offer various Custom Bioinformatics Solutions, including new tool and pipeline developments, by using state-of-the-art algorithms and computational methods. With our personal and customized approach, we deliver most reliable and accurate outcomes enabling you to differentiate and drive innovation. Post-project, we provide detailed documentation and reporting, as well as secure and full transfer of custom pipeline.
DNA Concentrator (Magnetic Beads)
The DNA Concentrator (Magnetic Beads) can be used to efficiently concentrate DNA/RNA from various samples with low concentration without the need of a DNA vacuum concentrator. Solid Phase Reversible Immobilization (SPRI) magnetic beads are well used for DNA and RNA purification and concentration. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to DNA and RNA.
The concentrator protocol is simple: mix Concentrator Buffer and Concentrator Beads with the sample, wash, and elute pure DNA in a small volume to concentrate the DNA samples. Moreover, the DNA/RNA samples are also purified during the procedure. The beads with our unique technology purify DNA/RNA samples effectively by removing unwanted components such as dNTPs, enzymes, detergents, proteins, and other contaminants.
DNA Normalization Magnetic Beads (NGS, PCR, gDNA)
The DNA Normalization Magnetic Beads (NGS, PCR, gDNA) was developed for normalization of NGS libraries, PCR fragments, sheared genomic DNA, and genomic DNA based on our magnetic bead technology. Magnetic beads has DNA-binding capacity. With the limitation of the DNA-binding capacity, normalized amount of DNA is recovered, and excess unbound DNA is separated.
The normalized DNA concentration can be obtained from DNA samples of various concentrations. Traditional fluorescent methods of DNA quantification require the setup of a standard curve from known DNA concentrations, compare with DNA samples from various sources, which would need extensive efforts and operating reagents. The protocol is simple, fast, centrifugation free, and filtration free. Additional DNA quantification and dilution is not necessary after DNA normalization with magnetic beads. Labor, time, and reagent are saved with the normalization beads.
The kit (Cat.# 40062) also includes unbound DNA recovery beads, which can be used for the recovery of unbound excess DNA during DNA normalization.
DNA Sequencing
Next-generation sequencing (NGS) technologies enable researchers to investigate the genetic basis of biological function, variation, and disease with increasing depth and throughput. Various DNA sequencing approaches are available to facilitate numerous applications, including variant discovery, genome assembly, resequencing, phylogenetics, genotyping, and taxon identification.
DNA sequencing services from Lexogen can start with DNA extraction or library preparation, with optional target enrichment. We use top-quality library generation methods with customized sequencing and data analysis approaches according to individual project requirements and target applications. DNA extraction can be performed upon request, e.g., from cell pellets, fresh or flash-frozen tissues, and FFPE curls*.
We also offer various RNA sequencing options if you are also interested in studying the transcriptome.
DNA Size Selection Kit (Magnetic Beads)
- Size range 50-100 bp
- Size range 100-200 bp
- Size range 200-500 bp
- Size range 250-350 bp (ideal for NGS library size selection)
- Size range 300-450 bp (ideal for NGS library size selection)
- Size range 450-750 bp (ideal for NGS library size selection)
- Size range 500-1000 bp
- Size range 1-3 kb
- Size range 1-5 kb
- Size range >5 kb (ideal for long-read sequencing size selection)
- Size range >10 kb (ideal for long-read sequencing size selection)
The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.