QuantSeq 3’ mRNA-Seq Library Prep Kit (REV) for Illumina with Custom Sequencing Primer
The QuantSeq REV Kit generates Illumina-compatible 3’ mRNA-Seq libraries directly targeting the 3’ end of polyadenylated transcripts. The first nucleotide of the read- out corresponds to the genuine transcription end site. The combination of cost-efficient 3’ mRNA sequencing and authentic end site representation makes QuantSeq REV the perfect tool to investigate alternative polyadenylation sites for projects at any scale.
Applications
- Alternative polyadenylation analysis
- Precise transcript end site mapping
- Gene expression analysis
Lexogen 12 nt UDIs included
QuantSeq 3’ mRNA-Seq V2 Library Prep Kit FWD with UDI 12 nt Set
Get the utmost quality out of our well-established QuantSeq 3’ mRNA-Seq gene expression kits, thanks to the improved V2 series! With the increasing reading capacity of next-generation sequencers, Lexogen’s patented 12 nt-long Unique Dual Indices will help you preserve most of your precious reads.
If you already have a library preparation kit without UDI (e.g., QuantSeq-Flex, Reverse, or Forward), our UDI Add-on V2 modules are available for purchase separately. They are delivered together with an improved PCR system, which can be seamlessly adapted to previous QuantSeq kits. For QuantSeq-Pool kits, you only need the UDI Sets (without purchasing additional PCR enzyme and buffer).
Our UDI Add-on V2 kits are also compatible with library preparation kits from other vendors.
QuantSeq-Pool Sample-Barcoded 3’ mRNA-Seq Library Prep Kit for Illumina, 96 preps
QuantSeq-Pool is the optimal solution for gene expression profiling for large screening projects using sample barcoding, early pooling, and batch processing of up to 96 samples in one reaction providing a workflow that is easily scalable for multiplexing up to 36,864 samples.
For multiplexing of more than 96 samples additional indexing is required. We recommend the Lexogen UDI 12 nt Sets (Cat. No. 101 – 105
