LUTHOR High-Definition Single-Cell 3′ mRNA-Seq Kit with UDI 12 nt Set B1
LUTHOR HD redefines the approach to single-cell RNA sequencing by offering an RNA-amplification-based kit able to detect virtually every single mRNA molecule present in the cell.
Like QuantSeq FWD, LUTHOR HD is a 3′ end mRNA-Seq kit that uses oligo(dT) primers to target polyadenylated RNAs and generate reads mapping to the 3′ end of transcripts. The two kits, however, use different protocols and technologies and are developed for different sample input amounts. While LUTHOR HD is designed for inputs ranging from 1 ng to 10 pg (equivalent to 100 cells to 1 cell) and even lower*, QuantSeq FWD is ideal for inputs ranging from 1 ng to 500 ng of total RNA.
LUTHOR HD is compatible with Lexogen’s patented 12 nt UDI Sets for maximal read rescue capabilities (no more misassigned or lost reads!).
* We have pushed the boundaries of sensitivity even further. LUTHOR HD enables to work with inputs as low as 1 pg, e.g. with cytoplasmic extracts
LUTHOR High-Definition Single-Cell 3′ mRNA-Seq Kit without UDI 12 nt Set
LUTHOR HD redefines the approach to single-cell RNA sequencing by offering an RNA-amplification-based kit able to detect virtually every single mRNA molecule present in the cell.
Like QuantSeq FWD, LUTHOR HD is a 3′ end mRNA-Seq kit that uses oligo(dT) primers to target polyadenylated RNAs and generate reads mapping to the 3′ end of transcripts. The two kits, however, use different protocols and technologies and are developed for different sample input amounts. While LUTHOR HD is designed for inputs ranging from 1 ng to 10 pg (equivalent to 100 cells to 1 cell) and even lower*, QuantSeq FWD is ideal for inputs ranging from 1 ng to 500 ng of total RNA.
LUTHOR HD is compatible with Lexogen’s patented 12 nt UDI Sets for maximal read rescue capabilities (no more misassigned or lost reads!).
* We have pushed the boundaries of sensitivity even further. LUTHOR HD enables to work with inputs as low as 1 pg, e.g. with cytoplasmic extracts
For full flexibility, these LUTHOR kits are delivered without the required indices. We recommend the Lexogen UDI 12 nt Sets (Cat. No. 101 – 105)
Poly(A) RNA Selection Kit V1.5, 96 preps
The Poly(A) RNA Selection Module enables the rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.
Optimized V1.5 Upgrade
The upgrade V1.5 contains new oligo(dT) Magnetic Beads (MB), compared to the V1.0 kit. The beads and solutions included in the V1.5 kit offer improved performance.
Highly Specific for Poly(A) RNA
Other RNA species (rRNA and tRNA) do not contain poly(A) sequences and therefore will not bind to the oligo(dT) beads.
Various Downstream Applications
Isolated mRNA can directly be used for RNA-Seq library preparation (e.g., CORALL RNA-Seq Library Prep kits), SAGE, CAGE, cloning, microarrays, cDNA synthesis, and others.
Rapid Turnaround
Polyadenylated RNAs can be isolated from total RNA samples within about one hour.
RiboCop rRNA Depletion Kit
Ceny dostępne dla zalogowanych UżytkownikówRiboCop rRNA Depletion Kits enable removal of ribosomal RNA from total RNA and are compatible with all random primed Library Prep Kits such as the CORALL RNA-Seq V2 Library Prep Kits (Cat. No. 171 – 176) for whole transcriptome sequencing.
RiboCop is available for various species:
- Human/Mouse/Rat (HMR, Cat. No. 144),
- Human/Mouse/Rat plus Globin (HMR+Globin, Cat. No. 145),
- Bacteria (Cat. No. 125 – 127), and
- Yeast (Cat. No. 190)
RiboCop kits are available as stand-alone kits in different sizes (24 and 96 preps).
For convenience, RiboCop for Human/Mouse/Rat (HMR or HMR+Globin) is also available as bundle with CORALL Total RNA-Seq Library Prep Kits. For optimal performance, we recommend CORALL Total RNA-Seq V2 Bundles with RiboCop rRNA depletion (Cat. No. 183 – 186).
RiboCop for Bacteria is available with three optimized Probe Mixes for depletion of rRNA from:
- mixed bacterial samples (META, Cat. No. 125),
- from Gram negative bacteria (G-, Cat. No. 126),
- or Gram positive bacteria (G+, Cat. No. 127).
New! RiboCop rRNA Depletion allows to unlock the complete yeast transcriptome and analyze non-coding RNAs.
Applications:
- Expression analysis, incl. non-coding transcripts
- Analysis of long non-coding RNA, snRNAs, snoRNAs, and circRNAs
- FFPE and Biobank transcriptomics
- Bacterial transcriptome research
- Metatranscriptomics
- Host-pathogen studies
- Yeast transcriptomics
Kit Content:
- Probe Mixes, Buffers, and Purification Beads
Small RNA-Seq Library Prep Kit for Illumina
- Gel-free user-friendly workflow
- Multiplexing of up to 96 samples
- Ready-to-sequence libraries in less than 5 hours
- Wide input range from 50 pg to 1,000 ng RNA
- Optimized for low RNA content samples such as plasma, serum, and urine
CORALL mRNA-Seq V2 Library Prep Kit with UDI 12 nt Set
Whole transcriptome mRNA sequencing is perfectly suited for expression analysis and delivers gene and transcript level quantification even for low input samples.
Get the best sequencing data with CORALL mRNA-Seq V2. The kit enables fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries with adjustable insert size.
CORALL mRNA-Seq V2 is a fast and flexible whole transcriptome sequencing kit with exceptional performance on low input RNA samples.
With Poly(A) Selection, including Lexogen 12 nt UDI Sets
CORALL Total RNA-Seq V2 Library Prep Kit with UDI 12 nt Set
Total RNA-Seq provides the optimal coverage for samples of varying quantity and quality and is perfectly suited for analyzing FFPE and Biobank samples, long non-coding RNA or perform bacterial transcriptomics after ribosomal RNA depletion.
Get the best sequencing data with CORALL Total RNA-Seq V2. The kit enables fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries with adjustable insert size. Bundles are available including rRNA depletion for human / mouse / rat ribosomal transcripts and globin mRNA for a convenient complete workflow solution.
Stand-alone Kits without rRNA Depletion, including Lexogen 12 nt UDI Sets
QuantSeq 3’ mRNA-Seq Library Prep Kit (REV) for Illumina with Custom Sequencing Primer
The QuantSeq REV Kit generates Illumina-compatible 3’ mRNA-Seq libraries directly targeting the 3’ end of polyadenylated transcripts. The first nucleotide of the read- out corresponds to the genuine transcription end site. The combination of cost-efficient 3’ mRNA sequencing and authentic end site representation makes QuantSeq REV the perfect tool to investigate alternative polyadenylation sites for projects at any scale.
Applications
- Alternative polyadenylation analysis
- Precise transcript end site mapping
- Gene expression analysis
Lexogen 12 nt UDIs included
QuantSeq 3’ mRNA-Seq V2 Library Prep Kit FWD with UDI 12 nt Set
Get the utmost quality out of our well-established QuantSeq 3’ mRNA-Seq gene expression kits, thanks to the improved V2 series! With the increasing reading capacity of next-generation sequencers, Lexogen’s patented 12 nt-long Unique Dual Indices will help you preserve most of your precious reads.
If you already have a library preparation kit without UDI (e.g., QuantSeq-Flex, Reverse, or Forward), our UDI Add-on V2 modules are available for purchase separately. They are delivered together with an improved PCR system, which can be seamlessly adapted to previous QuantSeq kits. For QuantSeq-Pool kits, you only need the UDI Sets (without purchasing additional PCR enzyme and buffer).
Our UDI Add-on V2 kits are also compatible with library preparation kits from other vendors.
QuantSeq-Pool Sample-Barcoded 3’ mRNA-Seq Library Prep Kit for Illumina, 96 preps
QuantSeq-Pool is the optimal solution for gene expression profiling for large screening projects using sample barcoding, early pooling, and batch processing of up to 96 samples in one reaction providing a workflow that is easily scalable for multiplexing up to 36,864 samples.
For multiplexing of more than 96 samples additional indexing is required. We recommend the Lexogen UDI 12 nt Sets (Cat. No. 101 – 105
RiboCop and CORALL Total RNA-Seq V2 Library Prep Kit with UDI 12 nt Set
Total RNA-Seq provides the optimal coverage for samples of varying quantity and quality and is perfectly suited for analyzing FFPE and Biobank samples, long non-coding RNA or perform bacterial transcriptomics after ribosomal RNA depletion.
Get the best sequencing data with CORALL Total RNA-Seq V2. The kit enables fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries with adjustable insert size. Bundles are available including rRNA depletion for human / mouse / rat ribosomal transcripts and globin mRNA for a convenient complete workflow solution.
CORALL Total RNA-Seq V2 Library Prep Bundles with RiboCop rRNA Depletion, including Lexogen 12 nt UDI Sets
