miRVEL Discovery Small RNA-Seq Library Prep Kit provides cutting-edge technology for sRNA-Seq, specifically optimized for low input biofluids samples, making it a perfect solution for circulating biomarker studies. The miRVEL Discovery technology utilizes modified oligonucleotides that effectively inhibit the formation of adapter dimers and eliminates the need for gel-size selection, thereby reducing hands-on time and streamlining the overall workflow. The kit accommodates a broad range of input total RNA, from as little as 1 ng to 500 ng, and is suitable for various sample types, including cells, tissues, exosomal RNA, plasma, serum, blood and other biofluids. The precise and unbiased quantification of sRNA is ensured by the incorporation of unique molecular identifiers (UMIs). Additionally, the kit minimizes the presence of hY4 Y RNA, which is often found at elevated levels in serum and plasma samples. 10 nt unique dual indices (UDIs) guarantee accurate read assignment and minimize the risk of read misassignment due to index hopping.

Features and Application:

• Discovery of novel biomarkers for cancer and other diseases
• Discovery and characterization of new sRNAs
• Mode-of-action for post-transcriptional regulation
• Drug discovery and target screens
• sRNA expression profiling
• Analysis of miRNA isoforms (isomiRs)

Kit Content:

• miRVEL Discovery Small RNA-Seq Library Prep Kit
• Purification Module for miRVEL Discovery Small RNA-Seq Library Prep KIt
• miRVEL Discovery UDI 10 nt Set

System Compatibility:

• • All Illumina sequencers
  • AVITI Cloudbreak