Benefits
- State-of-the-art, high-throughput scRNA-Seq
- Processing various tissue types and cell suspensions
- Pilot experiments for proper optimization offered
- In-depth data analysis available
Deliverables
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Cell ranger basic analysis: annotation with cellranger mkref, cell ranger count, clustering, differential gene expression, PCA, T-SNE, UMAP embeddings
- more advanced analysis or outputs is available, e.g., Doublet detection, processing with Seurat pipeline, heatmaps, trajectory inference etc.
Application-specific Documents
Applications
- Reveal the uniqueness of the individual cells to explore novel targets and biomarkers
- Explore cell heterogeneity
- Study differentiation and development on a cellular level
- Follow disease and treatment progress on a cellular level
- Explore cellular networks
- Discover novel cell populations
Resources
General Services Documents
Sample submission
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- RNA extraction from tissues, cells, and biofluids.
- Challenging samples such as FFPE curls, microdissections, plant tissues and similar.
- Sample QC included.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
Working with us
We offer different NGS Services packages. You can send us samples from which we will extract RNA (Start from Extraction) or purified RNA (Start from Library Prep). It is also possible to do “Sequencing only” projects of your prepared and pooled libraries, all library types are expected. Each service package can be further customized based on your sample type and requirements. Data Analysis is offered for each of our NGS services packages or as a service on its own (Lexogen Bioinformatics Services). We are also specialized in Custom Bioinformatics Solutions, e.g., for new pipeline developments.