SPRI (Solid Phase Reversible Immobilization) beads are often used for DNA purification because they are simple, fast, and effective. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
We have developed SPRI Beads (microRNA & Oligo Purification). Our proprietary bead technology overcomes the hurdle of the short DNA/RNA recovery problem. The beads purify short DNA/RNA purification effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The SPRI bead reagents are RNase free, and can be used for both DNA and RNA applications.
Our SPRI beads are optimized to selectively purify microRNA and DNA/RNA fragments that are as short as 20 bases. Purified short DNA and RNA fragments are ideal for applications requiring high quality fragments, as the fragments are free of impurities and contaminants.
Comparison of short DNA fragments recovery. BioDynami 20 bp DNA ladder (Cat. # 10002 )was used as DNA input. Ampure XP beads, BioDynami beads (DNA & RNA purification) and BioDynami beads (microRNA & Oligo purification) were tested. Only BioDynami beads (microRNA & Oligo purification) successfully recovered DNA fragments between 20-100 bp.
Features
- Effective purification of short DNA and RNA samples
- microRNA
- dsDNA fragments 20 bp or longer
- ssDNA fragments 20 nt or longer
- RNA fragments 20 nt or longer
- DNA/RNA hybrid fragments 20 bp or longer
- Oligo and chimeric oligo 20 nt or longer
- Removal of impurities and unwanted reaction components
- Compatibility: Works with both manual and automated procedures