Benefits
- Ultra-low input: 10 pg to 1 ng of total RNA*, corresponding to 1-100 cells.
- Supreme sensitivity results in more detected genes than other high-throughput technologies.
- Detection of lowly expressed transcripts (<10 copies/cell) possible
- No more gDNA in your reads, thanks to our proprietary technology
- We also accept low-quality RNA
*Our technology enables us to work with inputs as low as 1 pg, e.g. with cytoplasmic extracts.
Deliverables
- Raw data
- Demultiplexed files (FASTQ)
- Trimmed files (FASTQ)
- Aligned files (BAM)
- Rseqc (.txt)
- Gene counts + counting summary (.tsv)
- Differential expression + DE summary (.tsv)
- Multiqc reports – raw, trimmed, alignment, rseqc (.html)
- Deliverables summarized in a PDF report
Applications
- High-definition single-cell differential gene expression analysis
- Post-bulk scRNA-Seq characterization of cell subpopulations
- Gene expression analysis of rare cell types or cells that are scarce, such as circulating tumor cells (CTCs) or innate lymphoid cells (ILCs).
- Subcellular (cytoplasm, organelle) RNA analysis
- Single nucleus RNA-Seq
- Gene expression analysis of low abundant transcript analysis
Resources
General Services Documents
Sample submission
Consultations
- Together we will identify the best experimental design for your project.
- Shipment support (such as help with customs and export documents), or organizing the shipment under certain conditions.
Sample Extraction
- RNA extraction from tissues, cells, and biofluids.
- Challenging samples such as FFPE curls, microdissections, plant tissues and similar.
- Sample QC included.
Library Preparation
- Various library preparation solutions, depending on and adapted to your specific experimental objectives.
- Library QC included.
Sequencing
- Read mode and read depth most suitable for your application.
Data Analysis and Report
- Data quality control.
- Data analysis in-house.
- Standard data analysis options, on-demand, custom-design data analyses, and custom pipeline development.
Working with us
We offer different NGS Services packages. You can send us samples from which we will extract RNA (Start from Extraction) or purified RNA (Start from Library Prep). It is also possible to do “Sequencing only” projects of your prepared and pooled libraries, all library types are expected. Each service package can be further customized based on your sample type and requirements. Data Analysis is offered for each of our NGS services packages or as a service on its own (Lexogen Bioinformatics Services). We are also specialized in Custom Bioinformatics Solutions, e.g., for new pipeline developments.